The dual role of p62 in ferroptosis of glioblastoma according to p53 status.

ABSTRACT:

Background

Ferroptosis plays a key role in human cancer, but its function and mechanism in glioma is not clear. P62/SQSTM1 was reported to inhibit ferroptosis via the activation of NRF2 signaling pathway. In this study we reveal a dual role of p62 in ferroptosis of glioblastoma (GBM) according to p53 status.

Method

Lipid peroxidation analysis, transmission electron microscopy (TEM), GSH assay were performed to determine the level of ferroptosis. Western blot and qPCR were obtained to detect the expression of ferroptosis markers. Construction of mutant plasmids, immunoprecipitation, luciferase assay and rescue-experiments were performed to explore the regulatory mechanism.

Results

P62 overexpression facilitates ferroptosis and inhibits SLC7A11 expression in p53 mutant

SUBMITTER: Yuan F 

PROVIDER: S-EPMC8881833 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature