A missing piece of the puzzle in pulmonary fibrosis: anoikis resistance promotes fibroblast activation.

ABSTRACT:

Background

Pulmonary fibrosis initiates a pneumonic cascade that leads to fibroblast dysfunction characterized by excess proliferation. Anoikis is a physiological process that ensures tissue development and homeostasis. Researchers have not clearly determined whether disruption of anoikis is involved in pulmonary fibrosis.

Results

Here, we investigated the mechanism by which silica induces fibroblast activation via anoikis resistance and subsequent fibrosis. Anoikis of lung fibroblasts, alveolar epithelial cells and endothelial cells during the process of fibrosis was detected using CCK-8, western blot, cell count and flow cytometry (FCM) assays. Although the three cell types showed similar increases in proliferation, the expression of NTRK2, a marker of anoikis resistance,

SUBMITTER: Yin J 

PROVIDER: S-EPMC8881884 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature