Synovial fluid proteome profile of surgical versus chemical induced osteoarthritis in rabbits.

ABSTRACT:

Background

Animal models are significant for understanding human osteoarthritis (OA). This study compared the synovial fluid proteomics changes in surgical and chemical induced OA models.

Methods

Thirty rabbits either had anterior cruciate ligament transection (ACLT) procedure or injected intra-articularly with monosodium iodoacetate (MIA, 8 mg) into the right knee. The joints were anatomically assessed, and the synovial fluid proteins analyzed using two-dimensional polyacrylamide gel electrophoresis (2DGE) and MALDI TOF/TOF mass spectrometry analysis at 4, 8 and 12 weeks. The proteins' upregulation and downregulation were compared with control healthy knees.

Results

Seven proteins (histidine-rich glycoprotein, beta-actin-like protein 2 isoform X1, retinol-binding pro

SUBMITTER: Syed Sulaiman SZ 

PROVIDER: S-EPMC8881915 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature