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Virus MIP-composites for SARS-CoV-2 detection in the aquatic environment.

ABSTRACT: SARS-CoV-2 is the virus responsible for causing the global COVID-19 pandemic. Identifying the presence of this virus in the environment could potentially improve the effectiveness of disease control measures. Environmental SARS-CoV-2 monitoring may become increasingly demanded in areas where the available testing methods are ineffective. In this study, we present an electrochemical polymer composites biosensor for measuring SARS-CoV-2 whole-virus particles in the environment. The sensitized layer was prepared from molecularly imprinted polymer (MIP) composites of inactivated SARS-CoV-2. Testing demonstrated increased sensor signaling with SARS-CoV-2 specifically, while lower responses were observed to the negative controls, H5N1 influenza A virus and non-imprinted polymers (NIPs). This sensor detected SARS-CoV-2 at concentrations as low as 0.1 fM in buffer and samples prepared from reservoir water with a 3 log-scale linearity.

SUBMITTER: Sukjee W

PROVIDER: S-EPMC8882038 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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Virus MIP-composites for SARS-CoV-2 detection in the aquatic environment.

Sukjee Wannisa W Thitithanyanont Arunee A Manopwisedjaroen Suwimon S Seetaha Supaphorn S Thepparit Chutima C Sangma Chak C

Materials letters 20220226

SARS-CoV-2 is the virus responsible for causing the global COVID-19 pandemic. Identifying the presence of this virus in the environment could potentially improve the effectiveness of disease control measures. Environmental SARS-CoV-2 monitoring may become increasingly demanded in areas where the available testing methods are ineffective. In this study, we present an electrochemical polymer composites biosensor for measuring SARS-CoV-2 whole-virus particles in the environment. The sensitized laye ...[more]

PMID: 35250128

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