Improved high-throughput screening technique to rapidly isolate Chlamydomonas transformants expressing recombinant proteins.

ABSTRACT: The single-celled eukaryotic green alga Chlamydomonas reinhardtii has long been a model system for developing genetic tools for algae, and is also considered a potential platform for the production of high-value recombinant proteins. Identifying transformants with high levels of recombinant protein expression has been a challenge in this organism, as random integration of transgenes into the nuclear genome leads to low frequency of cell lines with high gene expression. Here, we describe the design of an optimized vector for the expression of recombinant proteins in Chlamydomonas, that when transformed and screened using a dual antibiotic selection, followed by screening using fluorescence activated cell sorting (FACS), permits rapid identification and isolation of microalgal transformants

SUBMITTER: Sproles AE 

PROVIDER: S-EPMC8882119 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature