Tumour cells are sensitised to ferroptosis via RB1CC1-mediated transcriptional reprogramming.

ABSTRACT:

Background

Ferroptosis, a form of regulated cell death, is an important topic in the field of cancer research. However, the signalling pathways and factors that sensitise tumour cells to ferroptosis remain elusive.

Methods

We determined the level of ferroptosis in cells by measuring cell death and lipid reactive oxygen species (ROS) production. The expression of RB1-inducible coiled-coil 1 (RB1CC1) and related proteins was analyzed by immunoblotting and immunohistochemistry. Immunofluorescence was used to determine the subcellular localization of RB1CC1. We investigated the mechanism of RB1CC1 nuclear translocation by constructing a series of RB1CC1 variants. To examine the ferroptosis- and RB1CC1-dependent transcriptional program in tumour cells, chromatin immunoprecipitati

SUBMITTER: Xue X 

PROVIDER: S-EPMC8882240 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature