Mobile CRISPR-Cas9 based anti-phage system in E. coli.

ABSTRACT: Escherichia coli is one of the most important microbial cell factories, but infection by bacteriophages in the environment may have a huge impact on its application in industrial production. Here, we developed a mobile CRISPR-Cas9 based anti-phage system for bacteriophages defense in E. coli. Two conjugative plasmids pGM1 (phosphoglucomutase 1) and pGM2 carrying one and two guide RNAs, respectively, were designed to defend against a filamentous phage. The results showed that the pGM1 and pGM2 could decrease the phage infection rate to 1.6% and 0.2% respectively in infected cells. For preventing phage infection in E. coli, the pGM2 decreased the phage infection rate to 0.1 %, while pGM1 failed to block phage infection. Sequence verification revealed that point mutations

SUBMITTER: Cao Z 

PROVIDER: S-EPMC8882345 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature