The Therapeutic Roles of Recombinant Hsp90α on Cornea Epithelial Injury.

ABSTRACT:

Purpose

The purpose of this study was to explore the therapeutic role of heat shock protein 90 (Hsp90) in wound healing of injury cornea epithelium.

Methods

The right eye of C57BL/6N male mice were performed the debridement wounds in the center of the cornea using an algerbrush II blade. The injured area was determined by staining the cornea with fluorescein sodium and measured with image-J. Immunoblotting, ELISA and immunochemistry were used for determining protein expression. The quantitation PCR was performed to measure mRNA expression.

Results

Hsp90α is upregulated at both the mRNA and protein levels, and is secreted extracellularly into the corneal stroma and tear film during the healing process after corneal injury in mice. This upregulation is associated with a

SUBMITTER: Wang M 

PROVIDER: S-EPMC8883155 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature