A human kinase yeast array for the identification of kinases modulating phosphorylation-dependent protein-protein interactions.

ABSTRACT: Protein kinases play an important role in cellular signaling pathways and their dysregulation leads to multiple diseases, making kinases prime drug targets. While more than 500 human protein kinases are known to collectively mediate phosphorylation of over 290,000 S/T/Y sites, the activities have been characterized only for a minor, intensively studied subset. To systematically address this discrepancy, we developed a human kinase array in Saccharomyces cerevisiae as a simple readout tool to systematically assess kinase activities. For this array, we expressed 266 human kinases in four different S. cerevisiae strains and profiled ectopic growth as a proxy for kinase activity across 33 conditions. More than half of the kinases showed an activity-dependent phenotype across many conditions an

SUBMITTER: Jehle S 

PROVIDER: S-EPMC8883442 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature