Tropheryma whipplei in the stool samples of children with acute diarrhea: a study from Tehran, Iran.

ABSTRACT:

Background

Recently, Tropheryma whipplei has been suggested as one of the causative agents of diarrhea among children worldwide. Limited data is available on the prevalence of T. whipplei among children with diarrhea in most countries such as Iran. This study was conducted to evaluate the prevalence of T. whipplei in children with acute diarrhea in Iran.

Methods

In this study, the stool samples were collected from 130 children under 10 years old with acute diarrhea from children's hospitals in Tehran city. Genomic DNA was extracted from stool samples and was tested for the presence of DNA of T. whipplei using the SYBR Green Real-time PCR method. Positive T. whipplei samples were finally confirmed by PCR Product sequencing.

Results

The mean age of participants was 32.5

SUBMITTER: Sayyahfar S 

PROVIDER: S-EPMC8883655 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature