Ultra-high sensitivity mass spectrometry quantifies single-cell proteome changes upon perturbation.

ABSTRACT: Single-cell technologies are revolutionizing biology but are today mainly limited to imaging and deep sequencing. However, proteins are the main drivers of cellular function and in-depth characterization of individual cells by mass spectrometry (MS)-based proteomics would thus be highly valuable and complementary. Here, we develop a robust workflow combining miniaturized sample preparation, very low flow-rate chromatography, and a novel trapped ion mobility mass spectrometer, resulting in a more than 10-fold improved sensitivity. We precisely and robustly quantify proteomes and their changes in single, FACS-isolated cells. Arresting cells at defined stages of the cell cycle by drug treatment retrieves expected key regulators. Furthermore, it highlights potential novel ones and allows cell

SUBMITTER: Brunner AD 

PROVIDER: S-EPMC8884154 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature