The discovery of novel coronaviruses in bat guano, Sarawak, Malaysian Borneo

ABSTRACT:

Purpose

Virological surveillance was conducted in to determine the presence of bat CoVs in the bat population in Wind Cave Nature Reserve, Sarawak, Malaysian Borneo.

Methods & Materials

Partial RNA dependent RNA polymerase (RdRp) gene sequences of 11 bat-CoV positive guano collected in Wind Cave Nature Reserve was analysed. Five pellets of fresh guano from insectivorous bats were collected on plastic tarp and pooled in 500uL of ice-cold viral transport medium. The 440-bp gene was amplified using family-wide hemi-nested RT-PCR for phylogenetic analysis. Amplicon sequences were analysed using Basic Local Alignment Search Tool software (BLAST) to determine their corresponding species. Sequence alignment was carried out without primer sequence and phylogenetic tree with Maxim

SUBMITTER: Tan C 

PROVIDER: S-EPMC8884765 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature