Rapid HILIC-Z ion mobility mass spectrometry (RHIMMS) method for untargeted metabolomics of complex biological samples.

ABSTRACT:

Introduction

Recent advances in high-throughput methodologies in the 'omics' and synthetic biology fields call for rapid and sensitive workflows in the metabolic phenotyping of complex biological samples.

Objective

The objective of this research was to evaluate a straightforward to implement LC-MS metabolomics method using a commercially available chromatography column that provides increased throughput. Reducing run time can potentially impact chromatography and therefore the effects of ion mobility spectrometry to expand peak capacity were also evaluated. Additional confidence provided via collision cross section measurements for detected features was also explored.

Methods

A rapid untargeted metabolomics workflow was developed with broad metabolome coverage, combin

SUBMITTER: Picmanova M 

PROVIDER: S-EPMC8885480 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature