Endogenous expression of Notch pathway molecules in human trabecular meshwork cells.

ABSTRACT:

Purpose

Cells in the trabecular meshwork sense and respond to a myriad of physical forces through a process known as mechanotransduction. Whilst the effect of substratum stiffness or stretch on TM cells have been investigated in the context of transforming growth factor (TGF-β), Wnt and YAP/TAZ pathways, the role of Notch signaling, an evolutionarily conserved pathway, recently implicated in mechanotransduction, has not been investigated in trabecular meshwork (TM) cells. Here, we compare the endogenous expression of Notch pathway molecules in TM cells from glaucomatous and non-glaucomatous donors, segmental flow regions, and when subjected to cyclical strain, or grown on hydrogels of varying rigidity.

Methods

Primary TM from glaucomatous (GTM), non-glaucomatous (NTM) donors

SUBMITTER: Dhamodaran K 

PROVIDER: S-EPMC8885976 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature