Microsampling with a Solid-Phase Extraction Cartridge: Storage and Online Mass Spectrometry Analysis.

ABSTRACT: This study aims to introduce the concept of utilizing a solid-phase extraction (SPE) cartridge for remote biofluid collection, followed by direct sample analysis at a later time. For this, a dried matrix spot was prepared in a syringe, in the form of SPE cartridge for the first time to enable small biofluid collection (microsampling), storage, shipment, and online electrospray ionization (ESI) mass spectrometry (MS) analysis of the stored dried samples. The SPE sorbents were packed into an ESI syringe and the resultant cartridge was used for sampling small volumes (<20 μL) of different complex biological fluids including blood, plasma, serum, and urine. The collected sample was stored in the dry state within the confinement of the SPE sorbent at room temperature, and analyte stability (

SUBMITTER: Kulyk DS 

PROVIDER: S-EPMC8886018 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature