Overexpression of interleukin-10 in engineered macrophages protects endothelial cells against LPS-induced injury in vitro.

ABSTRACT: Endothelial dysfunction is a primary pathophysiological change in sepsis. Macrophages are known to interact with vascular endothelial cells during the development of sepsis. Recently, drug delivery based on engineered macrophages was reported as an alternative approach for the management of diseases. Interleukin-10 (IL10) is a well-known anti-inflammatory cytokine, which reduces inflammation and inhibits dysfunction of endothelial cells caused by sepsis. It is currently poorly understood whether genetically modified macrophages with overexpression of IL10 are able to restore endothelial integrity and function at the cellular level. In this study, we used lentiviral vectors to construct RAW264.7 macrophages engineered to overexpress IL10 (IL10-eM) and investigated the effects of the IL10-eM

SUBMITTER: Yi L 

PROVIDER: S-EPMC8886523 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature