N⁶ -methyladenosine (m⁶ A) RNA modification of G protein-coupled receptor 133 increases proliferation of lung adenocarcinoma.

ABSTRACT: Lung adenocarcinoma (LUAD) accounts for almost 40% of lung cancers, leading to significant associated morbidity and mortality rates. However, the mechanism of LUAD tumorigenesis remains far from clear. Here, we scanned down-regulated genes involved in LUAD sourced from The Cancer Genome Atlas and Gene Expression Omnibus data and focused on G protein-coupled receptor 133 (GPR133). We offer compelling evidence that GPR133 was expressed at low levels in the setting of LUAD, and higher expression was positively related to a better prognosis among patients with LUAD. Functionally, GPR133 inhibited cell proliferation and tumor growth in vitro and in vivo. Regarding the mechanism, flow cytometry assays and western blot assays showed that GPR133 enhanced p21 and decreased cyclin B1 expression, thu

SUBMITTER: Wu G 

PROVIDER: S-EPMC8886537 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature