Homozygous Mutation of gsdf Causes Infertility in Female Nile Tilapia (Oreochromis niloticus).

ABSTRACT: Gonadal somatic cell-derived factor (Gsdf) is a member of the TGF-β superfamily, which exists mainly in fishes. Homozygous gsdf mutations in Japanese medaka and zebrafish resulted in infertile females, and the reasons for their infertility remain unknown. This study presents functional studies of Gsdf in ovary development using CRISPR/Cas9 in Nile tilapia (Oreochromis niloticus). The XX wild type (WT) female fish regularly reproduced from 12 months after hatching (mah), while the XX gsdf^-/- female fish never reproduced and were infertile. Histological observation showed that at 24 mah, number of phase IV oocyte in the XX gsdf^-/- female fish was significantly lower than that of the WT fish, although their gonadosomatic index (GSI) was simila

SUBMITTER: Jiang DN 

PROVIDER: S-EPMC8886716 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature