Generation of a BAC transgenic mouse strain that expresses CreERT and a fluorescent protein under the transcriptional control of the Fzd5 locus.

ABSTRACT:

Background

The expression of FZD5 distinguishes immature human mesenchymal stem/stromal cells (MSC) in cultures, and the function of FZD5 is crucial for maintaining the proliferation and multilineage differentiation capacity of human MSC. We herein investigated whether Fzd5 expression also marks undifferentiated MSC in animals.

Methods

We generated a transgenic mouse strain (Fzd5-CreERT-tFP635) that expresses CreERT and the fluorescent protein, TurboFP635 (tFP635), under the transcriptional control of the Fzd5 gene using the BAC transgenic technique, and identified cells expressing tFP635 by flow cytometry. We also conducted lineage tracing with this strain.

Results

In the bone marrow of transgenic mice, tFP635 was preferentially expressed in MSC, Leptin receptor-expr

SUBMITTER: Miyagi S 

PROVIDER: S-EPMC8886790 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature