RNA modifications stabilize the tertiary structure of tRNAfMet by locally increasing conformational dynamics.

ABSTRACT: A plethora of modified nucleotides extends the chemical and conformational space for natural occurring RNAs. tRNAs constitute the class of RNAs with the highest modification rate. The extensive modification modulates their overall stability, the fidelity and efficiency of translation. However, the impact of nucleotide modifications on the local structural dynamics is not well characterized. Here we show that the incorporation of the modified nucleotides in tRNAfMet from Escherichia coli leads to an increase in the local conformational dynamics, ultimately resulting in the stabilization of the overall tertiary structure. Through analysis of the local dynamics by NMR spectroscopic methods we find that, although the overall thermal stability of the tRNA is higher for the modified molecule, th

SUBMITTER: Biedenbander T 

PROVIDER: S-EPMC8887418 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature