Transcription-replication coordination revealed in single live cells.

ABSTRACT: The coexistence of DNA replication and transcription during S-phase requires their tight coordination to prevent harmful conflicts. While extensive research revealed important mechanisms for minimizing these conflicts and their consequences, little is known regarding how the replication and transcription machinery are coordinated in real-time. Here, we developed a live-cell imaging approach for the real-time monitoring of replisome progression and transcription dynamics during a transcription-replication encounter. We found a wave of partial transcriptional repression ahead of the moving replication fork, which may contribute to efficient fork progression through the transcribed gene. Real-time detection of conflicts revealed their negative impact on both processes, leading to fork stallin

SUBMITTER: Tsirkas I 

PROVIDER: S-EPMC8887460 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature