Synthetic robust perfect adaptation achieved by negative feedback coupling with linear weak positive feedback.

ABSTRACT: Unlike their natural counterparts, synthetic genetic circuits are usually fragile in the face of environmental perturbations and genetic mutations. Several theoretical robust genetic circuits have been designed, but their performance under real-world conditions has not yet been carefully evaluated. Here, we designed and synthesized a new robust perfect adaptation circuit composed of two-node negative feedback coupling with linear positive feedback on the buffer node. As a key feature, the linear positive feedback was fine-tuned to evaluate its necessity. We found that the desired function was robustly achieved when genetic parameters were varied by systematically perturbing all interacting parts within the topology, and the necessity of the completeness of the topological structures was ev

SUBMITTER: Sun Z 

PROVIDER: S-EPMC8887471 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature