Ptf1a Promoter-Driven Cre Expression During Spermatogenesis Causes Germline Recombination.

ABSTRACT:

Objectives

Two independently developed Ptf1a-Cre mouse lines, Ptf1atm1(cre)Hnak and Ptf1atm1(Cre)Cvw, are widely used in pancreatic research. Recently, Ptf1atm1(cre)Hnak line was reported to transmit unwanted paternal recombination. We aimed to investigate whether this exists in the Ptf1atm1(Cre)Cvw line.

Methods

Ptf1atm1(Cre)Cvw mice were crossed with R26-LSL-LacZ reporter mice. DNA recombination and gene expression were examined by recombination-specific polymerase chain reaction, reverse transcription-polymerase chain reaction, and X-Gal staining.

Results

R26 locus recombination was detected in the pancreas as well as the testes and sperm of the double transgenic mice. Positive ptf1a mRNA expression from testes revealed that there was endogenous Ptf1a promoter acti

SUBMITTER: Wang J 

PROVIDER: S-EPMC8887793 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature