Protective mechanism of dried blood spheroids: stabilization of labile analytes in whole blood, plasma, and serum.

ABSTRACT: Three-dimensional (3D) dried blood spheroids form when whole blood is deposited onto hydrophobic paper and allowed to dry in ambient air. The adsorbed 3D dried blood spheroid present at the surface of the hydrophobic paper is observed to offer enhanced stability for labile analytes that would otherwise degrade if stored in the traditional two-dimensional (2D) dried blood spot method. The protective mechanism for the dried blood spheroid microsampling platform was studied using scanning electron microscopy (SEM), which revealed the presence of a passivation thin film at the surface of the spheroid that serves to stabilize the interior of the spheroid against environmental stressors. Through time-course experiments based on sequential SEM analyses, we discovered that the surface protective t

SUBMITTER: Frey BS 

PROVIDER: S-EPMC8887831 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature