CircRNA PRH1-PRR4 stimulates RAB3D to regulate the malignant progression of NSCLC by sponging miR-877-5p.

ABSTRACT:

Background

Previous reports have confirmed the importance of circular RNA (circRNA) in the malignant progression of non-small-cell lung cancer (NSCLC). However, the role of circRNA PRH1-PRR4 readthrough (circPRH1-PRR4) in NSCLC progression was unclear. This study was designed to reveal the mechanism behind circPRH1-PRR4 regulating NSCLC progression.

Methods

Quantitative real-time polymerase chain reaction and western blot were employed to detect the expression of circPRH1-PRR4, microRNA-877-5p (miR-877-5p), the member RAS oncogene family (RAB3D), and other indicated protein markers. The positive expression rate of RAB3D was detected by immunohistochemistry assay. Cell proliferation was investigated by cell colony formation and 5-ethynyl-2'-deoxyuridine assays. Flow cytometry

SUBMITTER: Ma J 

PROVIDER: S-EPMC8888154 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature