Multi-modal profiling of human fetal liver hematopoietic stem cells reveals the molecular signature of engraftment.

ABSTRACT: The human hematopoietic stem cell harbors remarkable regenerative potential that can be harnessed therapeutically. During early development, hematopoietic stem cells in the fetal liver undergo active expansion while simultaneously retaining robust engraftment capacity, yet the underlying molecular program responsible for their efficient engraftment remains unclear. Here, we profile 26,407 fetal liver cells at both the transcriptional and protein level including ~7,000 highly enriched and functional fetal liver hematopoietic stem cells to establish a detailed molecular signature of engraftment potential. Integration of transcript and linked cell surface marker expression reveals a generalizable signature defining functional fetal liver hematopoietic stem cells and allows for the stratificat

SUBMITTER: Vanuytsel K 

PROVIDER: S-EPMC8888592 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature