Optimization of cerebrospinal fluid microbial DNA metagenomic sequencing diagnostics.

ABSTRACT: Infection in the central nervous system is a severe condition associated with high morbidity and mortality. Despite ample testing, the majority of encephalitis and meningitis cases remain undiagnosed. Metagenomic sequencing of cerebrospinal fluid has emerged as an unbiased approach to identify rare microbes and novel pathogens. However, several major hurdles remain, including establishment of individual limits of detection, removal of false positives and implementation of universal controls. Twenty-one cerebrospinal fluid samples, in which a known pathogen had been positively identified by available clinical techniques, were subjected to metagenomic DNA sequencing. Fourteen samples contained minute levels of Epstein-Barr virus. The detection threshold for each sample was calculated by usin

SUBMITTER: Olausson J 

PROVIDER: S-EPMC8888594 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature