Temporal and spatial cellular and molecular pathological alterations with single-cell resolution in the adult spinal cord after injury.

ABSTRACT: Spinal cord injury (SCI) involves diverse injury responses in different cell types in a temporally and spatially specific manner. Here, using single-cell transcriptomic analyses combined with classic anatomical, behavioral, electrophysiological analyses, we report, with single-cell resolution, temporal molecular and cellular changes in crush-injured adult mouse spinal cord. Data revealed pathological changes of 12 different major cell types, three of which infiltrated into the spinal cord at distinct times post-injury. We discovered novel microglia and astrocyte subtypes in the uninjured spinal cord, and their dynamic conversions into additional stage-specific subtypes/states. Most dynamic changes occur at 3-days post-injury and by day-14 the second wave of microglial activation emerged, a

SUBMITTER: Li C 

PROVIDER: S-EPMC8888618 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature