Compounds enhancing human sperm motility identified using a high-throughput phenotypic screening platform.

ABSTRACT:

Study question

Can a high-throughput screening (HTS) platform facilitate male fertility drug discovery?

Summary answer

An HTS platform identified a large number of compounds that enhanced sperm motility.

What is known already

Several efforts to find small molecules modulating sperm function have been performed but none have used high-throughput technology.

Study design, size, duration

Healthy donor semen samples were used and samples were pooled (3-5 donors per pool). Primary screening was performed singly; dose-response screening was performed in duplicate (using independent donor pools).

Participants/materials, setting, methods

Spermatozoa isolated from healthy donors were prepared by density gradient centrifugation and incubated in 384-well plates wit

SUBMITTER: Gruber FS 

PROVIDER: S-EPMC8888995 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature