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ABSTRACT: Objectives
There is a lack of effective biomarkers for neurodegenerative diseases (NDs) such as Alzheimer's disease (AD), Parkinson's disease (PD), amyotrophic lateral sclerosis (ALS), and frontotemporal dementia. Extracellular vesicle (EV) RNA cargo can have an interesting potential as a non-invasive biomarker for NDs. However, the knowledge about the abundance of EV-mRNAs and their contribution to neurodegeneration is not clear.Methods
Large and small EVs (LEVs and SEVs) were isolated from plasma of patients and healthy volunteers (control, CTR) by differential centrifugation and filtration, and RNA was extracted. Whole transcriptome was carried out using next generation sequencing (NGS).Results
Coding RNA (i.e., mRNA) but not long non-coding RNAs (lncRNAs) in SEVs and LEVs of patients with ALS could be distinguished from healthy CTRs and from other NDs using the principal component analysis (PCA). Some mRNAs were found in commonly deregulated between SEVs of patients with ALS and frontotemporal dementia (FTD), and they were classified in mRNA processing and splicing pathways. In LEVs, instead, one mRNA and one antisense RNA (i.e., MAP3K7CL and AP003068.3) were found to be in common among ALS, FTD, and PD. No deregulated mRNAs were found in EVs of patients with AD.Conclusion
Different RNA regulation occurs in LEVs and SEVs of NDs. mRNAs and lncRNAs are present in plasma-derived EVs of NDs, and there are common and specific transcripts that characterize LEVs and SEVs from the NDs considered in this study.
SUBMITTER: Sproviero D
PROVIDER: S-EPMC8889100 | biostudies-literature | 2022
REPOSITORIES: biostudies-literature
Sproviero Daisy D Gagliardi Stella S Zucca Susanna S Arigoni Maddalena M Giannini Marta M Garofalo Maria M Fantini Valentina V Pansarasa Orietta O Avenali Micol M Ramusino Matteo Cotta MC Diamanti Luca L Minafra Brigida B Perini Giulia G Zangaglia Roberta R Costa Alfredo A Ceroni Mauro M Calogero Raffaele A RA Cereda Cristina C
Frontiers in aging neuroscience 20220216
<h4>Objectives</h4>There is a lack of effective biomarkers for neurodegenerative diseases (NDs) such as Alzheimer's disease (AD), Parkinson's disease (PD), amyotrophic lateral sclerosis (ALS), and frontotemporal dementia. Extracellular vesicle (EV) RNA cargo can have an interesting potential as a non-invasive biomarker for NDs. However, the knowledge about the abundance of EV-mRNAs and their contribution to neurodegeneration is not clear.<h4>Methods</h4>Large and small EVs (LEVs and SEVs) were i ...[more]