Generation of Cortical Brain Organoid with Vascularization by Assembling with Vascular Spheroid.

ABSTRACT:

Background and objectives

Brain organoids have the potential to improve our understanding of brain development and neurological disease. Despite the importance of brain organoids, the effect of vascularization on brain organoids is largely unknown. The objective of this study is to develop vascularized organoids by assembling vascular spheroids with cerebral organoids.

Methods and results

In this study, vascularized spheroids were generated from non-adherent microwell culture system of human umbilical vein endothelial cells, human dermal fibroblasts and human umbilical cord blood derived mesenchymal stem cells. These vascular spheroids were used for fusion with iPSCs induced cerebral organoids. Immunostaining studies of vascularized organoids demonstrated well organized vasc

SUBMITTER: Kook MG 

PROVIDER: S-EPMC8889335 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature