Development and application of a reverse-transcription recombinase-aided amplification assay for subgroup J Avian leukosis virus.

ABSTRACT: Subgroup J Avian leukosis virus (ALV-J) is an important pathogen of poultry tumor diseases. Since its discovery, it has caused significant economic losses to the poultry industry. Thus, the rapid detection of molecular level with strong specificity is particularly important whether poultry are infected with ALV-J. In this study, we designed primers and probe for real-time fluorescent reverse-transcription recombinase-aided amplification assay (RT-RAA) based on the ALV-J gp85 sequence. We had established a real-time fluorescent RT-RAA method and confirmed this system by verifying the specificity and sensitivity of the primers and probe. In addition, repeatability tests and clinical sample regression tests were used for preliminary evaluation of this detection method. The sensitivity of esta

SUBMITTER: Wu XH 

PROVIDER: S-EPMC8889409 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature