Cascaded Enzyme Reactions over a Three-Dimensional, Wireframe DNA Origami Scaffold.

ABSTRACT: DNA nanotechnology has increasingly been used as a platform to scaffold enzymes based on its unmatched ability to structure enzymes in a desired format. The capability to organize enzymes has taken many forms from more traditional 2D pairings on individual scaffolds to recent works introducing enzyme organizations in 3D lattices. As the ability to define nanoscale structure has grown, it is critical to fully deconstruct the impact of enzyme organization at the single-scaffold level. Here, we present an open, three-dimensional (3D) DNA wireframe octahedron which is used to create a library of spatially arranged organizations of glucose oxidase and horseradish peroxidase. We explore the contribution of enzyme spacing, arrangement, and location on the 3D scaffold to cascade activity. The expe

SUBMITTER: Kahn JS 

PROVIDER: S-EPMC8889550 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature