IL-10 Indirectly Downregulates IL-4-Induced IgE Production by Human B Cells.

ABSTRACT: Controlled allergic disease is associated with decreased allergen-specific IgE and increased allergen-specific IgG4. Although IL-10 has been shown to contribute to these changes, the underlying mechanisms are largely unknown. This study explored how IL-10 differentially regulates human IgE and IgG4 production. Highly purified B cells and B cell subsets from healthy individuals were cultured with combinations of anti-CD40, IL-4, and IL-10. In other experiments, PBMCs isolated from healthy donors or from autosomal dominant hyper-IgE syndrome (STAT3 loss-of-function) subjects were cultured with combinations of IL-4 and IL-10. In B cell cultures, IL-10 had no significant effect on IL-4-induced IgE production but increased IL-4-induced IgG4 production over 20-fold. IL-4-induced transcription of

SUBMITTER: Lin AA 

PROVIDER: S-EPMC8890443 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature