Dynamic RNA Fitness Landscapes of a Group I Ribozyme during Changes to the Experimental Environment.

ABSTRACT: Fitness landscapes of protein and RNA molecules can be studied experimentally using high-throughput techniques to measure the functional effects of numerous combinations of mutations. The rugged topography of these molecular fitness landscapes is important for understanding and predicting natural and experimental evolution. Mutational effects are also dependent upon environmental conditions, but the effects of environmental changes on fitness landscapes remains poorly understood. Here, we investigate the changes to the fitness landscape of a catalytic RNA molecule while changing a single environmental variable that is critical for RNA structure and function. Using high-throughput sequencing of in vitro selections, we mapped a fitness landscape of the Azoarcus group I ribozyme under eight d

SUBMITTER: Peri G 

PROVIDER: S-EPMC8890501 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature