N⁶-Methyladenosine Modification Profile in Bovine Mammary Epithelial Cells Treated with Heat-Inactivated Staphylococcus aureus.

ABSTRACT: The symptoms of mastitis caused by Staphylococcus aureus (S. aureus) in dairy cows are not obvious and difficult to identify, resulting in major economic losses. N⁶-Methyladenosine (m⁶A) modification has been reported to be closely associated with the occurrence of many diseases. However, only a few reports have described the role of m⁶A modification in S. aureus-induced mastitis. In this study, after 24 h of treatment with inactivated S. aureus, MAC-T cells (an immortalized bovine mammary epithelial cell line) showed increased expression levels of the inflammatory factors IL-1β, IL-6, TNF-α, and reactive oxygen species. We found that the mRNA levels of METLL3, METLL14, WTAP, and ALKBH5 were also upregulated. Methyl

SUBMITTER: Li T 

PROVIDER: S-EPMC8890870 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature