Secondhand smoke affects reproductive functions by altering the mouse testis transcriptome, and leads to select intron retention in Pde1a.

ABSTRACT:

Background

Human exposure to secondhand smoke (SHS) is known to result in adverse effects in multiple organ systems. However, the impact of SHS on the male reproductive system, particularly on the regulation of genes and molecular pathways that govern sperm production, maturation, and functions remains largely understudied.

Objective

We investigated the effects of SHS on the testis transcriptome in a validated mouse model.

Methods

Adult male mice were exposed to SHS (5 h/day, 5 days/week for 4 months) as compared to controls (clean air-exposed). RNA-seq analysis was performed on the testis of SHS-exposed mice and controls. Variant discovery and plink association analyses were also conducted to detect exposure-related transcript variants in SHS-treated mice.

Results

SUBMITTER: Tommasi S 

PROVIDER: S-EPMC8891074 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature