The Challenge of Long-Term Cultivation of Human Precision-Cut Lung Slices.

ABSTRACT: Human precision-cut lung slices (PCLS) have proven to be an invaluable tool for numerous toxicologic, pharmacologic, and immunologic studies. Although a cultivation period of <1 week is sufficient for most studies, modeling of complex disease mechanisms and investigating effects of long-term exposure to certain substances require cultivation periods that are much longer. So far, data regarding tissue integrity of long-term cultivated PCLS are incomplete. More than 1500 human PCLS from 16 different donors were cultivated under standardized, serum-free conditions for up to 28 days and the viability, tissue integrity, and the transcriptome was assessed in great detail. Even though viability of PCLS was well preserved during long-term cultivation, a continuous loss of cells was observed. Altho

SUBMITTER: Preuß EB 

PROVIDER: S-EPMC8891143 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature