Short-read and long-read full-length transcriptome of mouse neural stem cells across neurodevelopmental stages.

ABSTRACT: During brain development, neural stem cells (NSCs) undergo multiple fate-switches to generate various neuronal subtypes and glial cells, exhibiting distinct transcriptomic profiles at different stages. However, full-length transcriptomic datasets of NSCs across different neurodevelopmental stages under similar experimental settings are lacking, which is essential for uncovering stage-specific transcriptional and post-transcriptional mechanisms underlying the fate commitment of NSCs. Here, we report the full-length transcriptome of mouse NSCs at five different stages during embryonic and postnatal development. We used fluorescent-activated cell sorting (FACS) to isolate CD133⁺Blbp⁺ NSCs from C57BL/6 transgenic mice that express enhanced green fluorescent protein (EGFP)

SUBMITTER: Ding C 

PROVIDER: S-EPMC8891264 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature