Contribution of the Mitochondrial Carbonic Anhydrase (MoCA1) to Conidiogenesis and Pathogenesis in Magnaporthe oryzae.

ABSTRACT: The interconversion of CO₂ and HCO₃ ^- catalyzed by carbonic anhydrases (CAs) is a fundamental biochemical process in organisms. During mammalian-pathogen interaction, both host and pathogen CAs play vital roles in resistance and pathogenesis; during planta-pathogen interaction, however, plant CAs function in host resistance but whether pathogen CAs are involved in pathogenesis is unknown. Here, we biologically characterized the Magnaporthe oryzae CA (MoCA1). Through detecting the DsRED-tagged proteins, we observed the fusion MoCA1 in the mitochondria of M. oryzae. Together with the measurement of CA activity, we confirmed that MoCA1 is a mitochondrial zinc-binding CA. MoCA1 expression, upregulated with H₂O₂

SUBMITTER: Dang Y 

PROVIDER: S-EPMC8891501 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature