Analysis of chromatin accessibility in p53 deficient spermatogonial stem cells for high frequency transformation into pluripotent state.

ABSTRACT:

Objectives

Spermatogonial stem cells (SSCs), the germline stem cells (GSCs) committed to spermatogenesis in niche, can transform into pluripotent state in long-term culture without introduction of exogenous factors, typically in p53 deficiency condition. As the guardian for genomic stability, p53 is associated with epigenetic alterations during SSCs transformation. However, the mechanism is still unknown, since complicated roles of p53 baffle our understanding of the regulating process.

Materials and methods

The chromatin accessibility and differentially expressed genes (DEGs) were analysed in p53^+/+ and p53^-/- SSCs using the Assay for Transposase-Accessible Chromatin with high-throughput Sequencing (ATAC-seq) and RNA-sequencing (RNA-seq), to explore th

SUBMITTER: Liu S 

PROVIDER: S-EPMC8891552 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature