ERp44 is required for endocardial cushion development by regulating VEGFA secretion in myocardium.

ABSTRACT:

Objectives

Endocardial cushions are precursors of the valve septum complex that separates the four heart chambers. Several genes have been implicated in the development of endocardial cushions. Specifically, ERp44 has been found to play a role in the early secretory pathway, but its function in heart development has not been well studied.

Materials and methods

In this study, we established conditional and tissue-specific knockout mouse models. The morphology, survival rate, the development of heart and endocardial cushion were under evaluation. The relationship between ERp44 and VEGFA was investigated by transcriptome, qPCR, WB, immunofluorescence and immunohistochemistry.

Results

ERp44 knockout (KO) mice were smaller in size, and most mice died during early postnatal

SUBMITTER: Bi Y 

PROVIDER: S-EPMC8891561 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature