In vivo development and single-cell transcriptome profiling of human brain organoids.

ABSTRACT:

Objectives

Human brain organoids can provide not only promising models for physiological and pathological neurogenesis but also potential therapies in neurological diseases. However, technical issues such as surgical lesions due to transplantation still limit their applications.

Materials and methods

Instead of applying mature organoids, we innovatively developed human brain organoids in vivo by injecting small premature organoids into corpus striatum of adult SCID mice. Two months after injection, single-cell transcriptome analysis was performed on 6131 GFP-labeled human cells from transplanted mouse brains.

Results

Eight subsets of cells (including neuronal cells expressing striatal markers) were identified in these in vivo developed organoids (IVD-organoids) by unb

SUBMITTER: Huang S 

PROVIDER: S-EPMC8891563 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature