Magnetic covalent organic framework nanospheres-based miRNA biosensor for sensitive glioma detection.

ABSTRACT: Sensitive detection and accurate diagnosis/prognosis of glioma remain urgent challenges. Herein, dispersed magnetic covalent organic framework nanospheres (MCOF) with uniformed Fe₃O₄ nano-assembly as cores and high-crystalline COF as shells were prepared by monomer-mediated in-situ interface growth strategy. Based on the unique interaction between MCOF and hairpin DNA, a fluorescent signal amplified miRNA biosensor was constructed. It could realize the sensitive detection of miRNA-182 in different matrixes, where the detection limit, linearity range and determination coefficient (R²) in real blood samples reached 20 fM, 0.1 pM-10 pM and 0.991, respectively. Also, it possessed good stability and precision as observed from the low intra-day/inter-day RSD and

SUBMITTER: Liang D 

PROVIDER: S-EPMC8892165 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature