Murine Model for Measuring Effects of Humanized-Dosing of Antibiotics on the Gut Microbiome.

ABSTRACT: There is a current need for enhancing our insight in the effects of antimicrobial treatment on the composition of human microbiota. Also, the spontaneous restoration of the microbiota after antimicrobial treatment requires better understanding. This is best addressed in well-defined animal models. We here present a model in which immune-competent or neutropenic mice were administered piperacillin-tazobactam (TZP) according to human treatment schedules. Before, during and after the TZP treatment, fecal specimens were longitudinally collected at established intervals over several weeks. Gut microbial taxonomic distribution and abundance were assessed through culture and molecular means during all periods. Non-targeted metabolomics analyses of stool samples using Quadrupole Time of Flight mas

SUBMITTER: Leopold SR 

PROVIDER: S-EPMC8892246 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature