Mini-XT, a miniaturized tagmentation-based protocol for efficient sequencing of SARS-CoV-2.

ABSTRACT:

Background

The COVID-19 pandemic has highlighted the importance of whole genome sequencing (WGS) of SARS-CoV-2 to inform public health policy. By enabling definition of lineages it facilitates tracking of the global spread of the virus. The evolution of new variants can be monitored and knowledge of specific mutations provides insights into the mechanisms through which the virus increases transmissibility or evades immunity. To date almost 1 million SARS-CoV-2 genomes have been sequenced by members of the COVID-19 Genomics UK (COG-UK) Consortium. To achieve similar feats in a more cost-effective and sustainable manner in future, improved high throughput virus sequencing protocols are required. We have therefore developed a miniaturized library preparation protocol with drastically

SUBMITTER: Fuchs M 

PROVIDER: S-EPMC8892412 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature