Glycosite-deleted mRNA of SARS-CoV-2 spike protein as a broad-spectrum vaccine.

ABSTRACT: Development of the messenger RNA (mRNA) vaccine has emerged as an effective and speedy strategy to control the spread of new pathogens. After vaccination, the mRNA is translated into the real protein vaccine, and there is no need to manufacture the protein in vitro. However, the fate of mRNA and its posttranslational modification inside the cell may affect immune response. Here, we showed that the mRNA vaccine of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein with deletion of glycosites in the receptor-binding domain (RBD) or especially the subunit 2 (S2) domain to expose more conserved epitopes elicited stronger antibody and CD8⁺ T cell responses with broader protection against the alpha, beta, gamma, delta, and omicron variants, compared to the unmo

SUBMITTER: Wu CY 

PROVIDER: S-EPMC8892489 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature