Label-free sensing of cells with fluorescence lifetime imaging: The quest for metabolic heterogeneity.

ABSTRACT: Molecular, morphological, and physiological heterogeneity is the inherent property of cells which governs differences in their response to external influence. Tumor cell metabolic heterogeneity is of a special interest due to its clinical relevance to tumor progression and therapeutic outcomes. Rapid, sensitive, and noninvasive assessment of metabolic heterogeneity of cells is a great demand for biomedical sciences. Fluorescence lifetime imaging (FLIM), which is an all-optical technique, is an emerging tool for sensing and quantifying cellular metabolism by measuring fluorescence decay parameters of endogenous fluorophores, such as NAD(P)H. To achieve accurate discrimination between metabolically diverse cellular subpopulations, appropriate approaches to FLIM data collection and analysis a

SUBMITTER: Shirshin EA 

PROVIDER: S-EPMC8892511 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature