Powerful, efficient QTL mapping in Drosophila melanogaster using bulked phenotyping and pooled sequencing.

ABSTRACT: Despite the value of recombinant inbred lines for the dissection of complex traits, large panels can be difficult to maintain, distribute, and phenotype. An attractive alternative to recombinant inbred lines for many traits leverages selecting phenotypically extreme individuals from a segregating population, and subjecting pools of selected and control individuals to sequencing. Under a bulked or extreme segregant analysis paradigm, genomic regions contributing to trait variation are revealed as frequency differences between pools. Here, we describe such an extreme quantitative trait locus, or extreme quantitative trait loci, mapping strategy that builds on an existing multiparental population, the Drosophila Synthetic Population Resource, and involves phenotyping and genotyping a populati

SUBMITTER: Macdonald SJ 

PROVIDER: S-EPMC8893256 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature