Spatially resolved metabolomic characterization of muscle invasive bladder cancer by mass spectrometry imaging.

ABSTRACT:

Introduction

Muscle invasive bladder cancer (MIBC) is an advanced stage of bladder cancer which poses a severe threat to life. Cancer development is usually accompanied by remarkable alterations in cell metabolism, and hence deep insights into MIBC at the metabolomic level can facilitate the understanding of the biochemical mechanisms involved in the cancer development and progression.

Methods

In this proof-of-concept study, the optimal cutting temperature (OCT)-embedded MIBC samples were first washed with pure water to remove the polymer compounds which could cause severe signal suppression during mass spectrometry. Further, the tissue sections were analyzed by infrared matrix-assisted laser desorption electrospray ionization mass spectrometry imaging (IR-MALDESI MSI), prov

SUBMITTER: Tu A 

PROVIDER: S-EPMC8893274 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature